Recombinant Humanized Type XVII Collagen promotes skin ageing and wound repair
INCI No. : WATER、MANNITOL、TREHALOSE、COLLAGEN
Place of Origin: Guangdong, China
Usage: Cosmetic Raw Materials, Anti-aging products, Moisturizers, Skin Repair Products
Product Names: Collagen type XVII
Appearance:
Lyophilized Powder:white or light yellow loose body, after adding sterile deionized water or PBS quickly re-soluble into a clear liquid.
Brand Name: NIKOO
Model Number: Collagen type XVII
Grade: Comestic Grade
Packaging Details: 100g/bag
Product Characteristics
Human type XVII collagen(COL 17A1 gene encoding) is a transmembrane non-fibroblast collagen, which is a homogeneous trimer composed of three identical α1 (XVII) chains and is divided into three major domains: intracellular, transmembrane, and
extracellular domains. Human type XVII collagen(COL XVII,also known as BP180) is a component of hemidesmosomes in cells, plays an important role in an interaction between epithelial cells and a basement membrane, can regulate the adhesion, separation, development,and differentiation of epithelial cells, and is important for the differentiation and regeneration of keratinocytes. Because
a content of human type XVII collagen in the human body is very low and a content of type XVII collagen in an animal
is also extremely low, type XVII collagen is very difficult to extract and cannot be mass-produced. Moreover, animalderived collagen products inevitably have immunogenicity and potential biological safety hazards such as viruses and epidemics, and thus are not widely used. Limited by legal and ethical constraints, human type XVII collagen can only be used in scientific research, and thus there are currently no commercial human-derived or animal-derived type XVII collagen products on the market, which further limits the research and application of human type XVII collagen. A main way for solving such problems now is to produce collagen through a biotechnology such as genetic engineering.
Recombinant human collagen XVII (RHCXVII) has emerged as a promising novel collagen material.Human collagen type XVII (HCXVII) plays an important role in regulating the adhesion, separation, development and differentiation of epithelial cells, which is crucial for the differentiation and regeneration of keratinocytes . However, natural COL XVII is present at low levels in the human body, which complicates its bulk extraction and large-scale production. Recombinant human collagen XVII (RHCXVII), a recombinant form of collagen XVII, has been developed as a synthetic single-chain protein that incorporates active fragments of the natural COL XVII.
RHCXVII exhibits several advantages over traditionally extracted animal collagen, including high purity, biocompatibility, low immunogenicity and abundant supply, making it a promising candidate for applications such as wound healing and protein replacement therapy
RHCXVII is composed of five domains (amino acid residues 567–619, 641–715, 906–938, 951–977, 1438–1482) of the α1 chain of HCXVII. The DNA encoding RHCXVII was obtained by chemical synthesis. A yeast expression vector containing the cDNA in pPIC9K was constructed, and the plasmids were digested with SacI to linearize the DNA for integration into the Komagataella phaffii GS115 genome. The selection of the recombinant engineered strains was conducted through a process of direct plating on YPD plates containing a high concentration of G418. Linked fed-batch fermentation was then performed in a 1000 L fermentation tank to obtain a fermentation mixture, which was purified by ion exchange chromatography to obtain high-purity (>95 %) RHCXVII.
Product Specification
|
Item |
Specification |
|
Product Grade |
Molecular biology grade, sterile lyophilized powder |
|
Product Names |
Recombinant Humanized Type XVII Collagen |
|
CAS Number |
9064-67-9 |
| Source Species | Microbial |
| Gene Source | Human |
| Product Specifications | 10g/bottle, 100g/bottle, etc. (customizable to kilogram-scale) |
| PI (Isoelectric Point) | 6.59 |
| PH Value(10% aqueous solution, 25℃) | 5-8 |
| Electrophoretic Purity | >95% (SDS-PAGE) |
| Protein Content | >90% |
| Dry Weight Loss | <10% |
| Thermal Stability | No foreign substances generated |
| Bacterial Endotoxin | <0.5EU/mg |
| Microorganisms | <5cfu/g |
|
Residual Heavy Metals
|
≥13% |
| Total bacterial cfu/g | <10ppm |
| Solubility/Water Solubility | Easily soluble in water |
| Shelf Life | Store at -20°C in a dry environment for at least 1 year after receipt |
|
Applications |
Collagen is an abundant protein in the human body, crucial for maintaining normal physiological functions. Recombinant humanized collagen, due to its excellent physical properties and biological characteristics, has widespread applications in pharmaceuticals, tissue engineering, food, cosmetics, and other fields. |
Product Application
Recombinant Humanized Type XVII Collagen is a transmembrane protein that forms hemidesmosomes. Recombinant Humanized Type XVII Collagen facilitates interactions between stem cells，surrounding cells，and the extracellular matrix，thereby regulating skin homeostasis，aging，and wound healing. Recombinant Humanized Type XVII Collagen also possesses unique cell adhesion sites and signal transduction functions，which can regulate cell migration，proliferation，and differentiation.
Recombinant Human Collagen Type XVII (COL17) is a purified Prokaryotic Protein. Purity: >97% by SDS-PAGE. Host: E. coli. Endotoxin Level: <0.5EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Collagen Type XVII (COL17). Target Synonyms: COL17A1; BP180; BPAG2; LAD-1; LABD97; Collagen Alpha-1(XVII)chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; Linear IgA disease antigen 1. Accession Number: Q9UMD9. Gene ID: 1308. Expression Region: Pro184~Pro375. Tag Info: N-terminal His Tag. Theoretical MW: 10-20/30-60kDa. Buffer: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. Storage: -20°C. Avoid repeated freeze/thaw cycles.
The recombinant human type XVII collagen can undergo efficient secretory and soluble expression in eukaryotic host cells such as Pichia pastoris (P. pastoris),and also exhibits more excellent cell adhesion activity,cell migration-promoting activity, tissue regeneration-promoting activity, and hair follicle repair and regeneration-promoting activity than commercial native human collagen
Type XVII collagen (COL17) is a transmembrane protein found in the epidermal basal layer, crucial for maintaining tight connections between the epidermis and dermis. It promotes epidermal turnover and stabilizes the basement membrane, thereby supporting anti-aging and repair processes from the basement membrane zone. Evidence suggests that the hydrolysis of COL17 can cause follicular miniaturization, ultimately leading to hair loss. Due to its unique physiological functions, COL17 shows promising potential in delaying and treating hair loss.
This product is recombinant humanized Type XVII collagen, derived from microorganisms. It boasts excellent water solubility, stability, hydrophilicity, and moisturizing properties. Utilizing the Pichia pastoris fermentation process, the product ensures lower endotoxin levels and greater safety.
Biological functions of collagen type XVII
Collagen type XVII is a transmembrane protein located primarily in the basement membrane region of the skin. It not only plays a role in maintaining the stability of the skin structure, but also plays an important supportive role in the function of pigment cells in the hair follicle.Deficiency or dysfunction of collagen type XVII leads to degradation of the hair follicle structure and may affect the survival and function of the pigment cells, which can lead to the formation of gray hair.
Mechanisms of action in the prevention of gray hair
Maintenance of pigment cell stability: By maintaining the integrity of the basement membrane region, type XVII collagen provides the microenvironment on which pigment cells depend for survival, thus preventing premature aging or apoptosis of pigment cells and reducing the appearance of gray hair.
Antioxidant protection: pigment cells are easily damaged under oxidative stress conditions, and collagen type XVII has certain antioxidant functions, which can help to resist the damage of oxidative stress on pigment cells and delay the formation of gray hair.
Regulation of cell signaling: Type XVII collagen may help maintain the natural color of hair by participating in cell signaling pathways that regulate pigment cell activity and differentiation.
Applications in gray to black hair conversion
Promote pigment cell regeneration: Collagen type XVII helps to activate dormant pigment stem cells in the hair follicle and promotes their differentiation into functional pigment cells, thus regenerating melanin in the formed gray hair and turning gray hair black.
Improve follicle microenvironment: By enhancing the health of the scalp and improving the microenvironment of the hair follicle, collagen type XVII supports the regeneration and functional restoration of pigment cells, which helps to reverse gray hair.
Slowing down the aging of hair follicles: By slowing down the aging process of hair follicles and pigment cells, collagen type XVII can slow down or reverse the loss of pigment in hair follicles, thus restoring the black color of hair to a certain extent.
Application Prospects
With in-depth research on type XVII collagen, its application in scalp care products is promising. Hair care products containing collagen type XVII, such as shampoos and serums, can be developed to help improve scalp health and promote hair regrowth. These products can be used in combination with other active ingredients, such as vitamins and plant extracts, to enhance the hair care effect.
Conclusion
Collagen type XVII shows great potential for use in scalp care. It plays an important role in improving scalp health and promoting hair regeneration by protecting the scalp barrier, promoting cell renewal and repair, and stimulating hair follicle stem cell activity. With advances in technology and more research, collagen type XVII has become an important ingredient in the field of scalp care.
Packaging
100g/bag according to customers' requirements
For samples, it is shipped by express , such as FedEx,DHL,TNT& UPS, it takes around 3-7 days. For bulk order, we will recommend to ship by sea. If you have cooperated shipping agent in China , we can ship to their warehouse for you.
If used for pure type III collagen lyophilized powder recommended additive amount: 10-50%, other types such as emulsions, creams, sprays and other products recommended additive amount: 0.1-5%.
It is recommended that its package is light-avoidance closed package to reduce contact with air and light sources, sealed storage, and pay attention to reasonable sun protection.
Storage: room temperature (5-25 ℃) to save sealed, shaded cool place to save, 4-5 ℃ refrigeration is preferred. After opening, wipe the bottle clean and store it in airtight refrigerated storage, it is recommended to use it up within 1 month after opening.
Shelf life: the product can remain stable for at least two years when stored in closed containers under the recommended conditions.
faq