Miraculous 5D-4500 five active collagen, gum raw material application of new wind direction
Five active collagen 5D-4500 (hereinafter referred to as: 5D-4500): the small intestinal submucosa extract (Small Intestinal Submucosa) as the raw material, decellularised tissue matrix composite material and its preparation method as the core technology (the technology has been awarded the national patent for invention), after enzymatic extraction, dialysis and purification of the preparation, it is to Ⅰ, Ⅲ, Ⅳ, VII collagen as the core, containing a small amount of fibronectin, a group of natural biological activity of the protein, mainly has a high repair activity, anti-aging and tighten the film and other functions.
Decellularised extracellular matrix (dECM) materials have received increasing attention due to their inherent structure, high bioactivity, low immunogenicity and good biodegradability, which are difficult to be imitated by synthetic materials. In recent years, dECM has been shown to have great potential in overcoming the shortcomings of autografts and is a promising material for tissue engineering. The extracellular matrix that has been processed by the decellularisation technique is known as decellularised matrix. Decellularised matrices are not only biocompatible, but also have a strong endogenous tissue-inducing capacity, which makes them ideally suited for cell adhesion and growth, as well as for regulating and accelerating the tissue repair process after implantation. Based on these advantages, decellularised matrices play an important role in filling the gap of tissue loss, inducing tissue regeneration, repairing damaged tissues, and completing the integration of surrounding tissues.
The biggest advantage of 5D-4500 collagen is that the microstructure of the active ingredient is preserved during the preparation process, which provides a guarantee for the repairing action. For example, the highest content of type I collagen in 5D-4500 maintains a complete triple helix structure in order to provide a fulcrum for cell attachment, transducing signals into the cells, promoting cell proliferation and migration, replacing and renewing broken cells, and completing the repair of the skin barrier.
5D-4500 collagen renews the cells through repair, and when the cells are repaired, they will naturally secrete intercellular lipids, thus completely solving the problem of damaged skin barrier, which is the biggest advantage and highlight of 5D-4500 collagen.
Type I collagen is fibrillar collagen
Type I collagen is the most abundant and commonly found fibrillar collagen in animals, accounting for about 90% of the total collagen in animals.
Type I collagen is mainly distributed in the skin, bones, tendons, ligaments, blood vessel walls and teeth, and is widely distributed in the interstitium of connective tissues, and is an important component of the interstitial tissues of some important organs in the body such as lungs, liver and kidneys. Type I collagen functions in the form of collagen fibres and has an impact on the physiological and pathological processes of cells, tissues and even the whole organism, in addition to acting as a tissue support to give tissue tension.
Type III collagen is fibrillar collagen
Type III collagen is widely found in connective tissues such as the skin, lungs, liver, intestines and vascular system, and is often distributed in the same locations as type I collagen. Type III collagen peptide chains contain cysteine, resulting in a small number of disulfide bonds between the peptide chains, which themselves can form fine fibres, whereas the covalent cross-linking bonds between the peptide chains of the other types of collagen are formed mainly by the side chains of lysine residues or hydroxylysine residues. Ultrastructural analysis of type III collagen mutant mice has shown that type III collagen is essential for the production of normal type I collagen fibres in the cardiovascular system, intestine and skin .
Type IV collagen reticular collagen
Type IV collagen is a non-fibrillar collagen (reticular collagen).
Type IV collagen is a major component of the basement membrane and exists in lamellar form. Type IV collagen is synthesised and metabolised mainly in the liver. In the liver, type IV collagen is found in the basement membrane around vascular endothelial cells, bile ducts and nerve fibres. It compensates for the function of the basement membrane of the skin and helps to bind the epidermis to the dermis, delivering water and nutrients to the dermis.
Type VII collagen is the anchoring collagen
Collagen type VII is mainly found in the skin, especially in the dermis. Many disorders in the synthesis and polymerisation of collagen may result in the absence or abnormal function of sequestrated protofibrils, leading to disruption of the dermal-epidermal junctions and the pathological changes of epidermolysis bullosa.Type VII collagen is essential for the function and stability of the extracellular matrix, and serves as an anchoring collagen that binds to types I and III collagen to provide stability to the structure of the interstitial and basement membranes.
FN fibronectin
FN is an important non-collagenous glycoprotein in the extracellular matrix and basement membrane, which plays a central role in cell adhesion, and is widely used in regenerative medicine as it participates in cell adhesion, spreading, proliferation, migration, and embryonic development during the body's life activities.FN has the role of a growth factor, which promotes cell proliferation, induces the formation of granulation tissue from the epidermal cells, and promotes the re-construction of the basement membrane under the epidermis and normal keratinisation process, as an important component of the skin extracellular matrix, which can rivet collagen tightly to the skin cells, strengthen the skin elastic fibre network, tighten the sagging skin, and promote skin cell growth. As an important component of the extracellular matrix of the skin, FN can rivet collagen onto the skin cells, strengthen the elastic fibre network of the skin, tighten the sagging skin, promote the growth, differentiation and migration of the skin cells, guide the skin to regenerate in an orderly manner, promote the expression of the barrier proteins, repair the skin barrier, and strengthen the skin's defences.
Five Active Collagen 5D-4500 Anti-Aging & Wrinkle Reduction
As we age, collagen secretion decreases, fibres break down, function diminishes and wrinkles deepen.
The triple helical structure of collagen binds to specific receptors on the cell membrane such as integrins and discoidal structural domain receptors (DDRs), inducing signal transduction through the cytoskeleton and regulating cell behaviour.
5D-4500 Collagen is a complex with collagen (Ⅰ, Ⅲ, Ⅳ, Ⅶ) and fibronectin as the main components, which has an extremely strong repair and anti-aging effect.
5D-4500 collagen scratch experiment conclusion: in 5D-4500 collagen concentration of 0.52ug/cm2 (5ppm) and collagen concentration of 5.2ug/cm2 (50ppm) are able to promote cell proliferation and migration.
Five active collagen 5D-4500 promote cell proliferation experiment - repair, anti-aging effect
Conclusion: 5D-4500 active collagen concentration of 0.025ug/cm2 (0.125ppm) can promote cell growth, the concentration of 1.6ug/cm2 (8ppm) to promote cell proliferation effect is obvious.
Five active collagen 5D-4500 other effects
Anti-aging: helps to improve cell microcirculation, promote skin metabolism and accelerate tissue regeneration.
Reduces scar formation: fills the interstitial space between cells, enhances intercellular structural support and lightens scars.
Moisturising: Collagen contains natural moisturising factors and hydrophilic groups, which can form a protective film on the skin surface and replenish skin moisture.
Reduces hyperpigmentation: tyrosine residues on collagen compete with tyrosine in the skin, effectively preventing melanin production.
Bactericidal: It can provide a slightly acidic environment (pH 4.5-5.5) to inhibit or kill bacteria directly.
Quintuple Active Collagen 5D-4500 Raw Material Viral Residue Test for Animal Sources
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The source of animals and animal feed is taken after strict quarantine, and the process of animals from birth to taking feed is strictly monitored to ensure that the raw materials used are not infected with disease-causing viruses and have relevant quarantine certificates;
5D-4500 collagen has been evaluated by an authoritative third-party testing organisation for the biological evaluation of medical devices, and the results of the 12-week animal experiments comply with the national standards, and third-party test reports can be provided;
5D-4500 collagen has been inactivated during the preparation process, and the verification of virus inactivation operation has been demonstrated by authoritative literature.
